Repeat region of Brugia malayi sheath protein (Shp-1) carries Dominant B epitopes recognized in filarial endemic population.
Identifieur interne : 002580 ( Main/Exploration ); précédent : 002579; suivant : 002581Repeat region of Brugia malayi sheath protein (Shp-1) carries Dominant B epitopes recognized in filarial endemic population.
Auteurs : Jeya Prita Parasurama Jawaharlal [Inde] ; Jayaprakasam Madhumathi ; Rajaiah Prabhu Prince ; Perumal KalirajSource :
- Acta parasitologica [ 1896-1851 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Protéines d'helminthes.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe.
- Animaux, Humains, Microfilaria, Test ELISA, Épitopes.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , immunology : Antigens, Helminth, Helminth Proteins.
- immunology : Brugia malayi, Elephantiasis, Filarial.
- parasitology : Elephantiasis, Filarial.
- Animals, Enzyme-Linked Immunosorbent Assay, Epitopes, Humans, Microfilariae.
Abstract
Transmission of lymphatic filariasis is mediated through microfilariae (L1 stage of the parasite) which is encased in an eggshell called sheath. The sheath protein Shp-1 stabilizes the structure due to the unique repeat region with Met-Pro-Pro-Gln-Gly sequences. Microfilarial proteins could be used as transmission blocking vaccines. Since the repeat region of Shp-1 was predicted to carry putative B epitopes, this region was used to analyze its reactivity with clinical samples towards construction of peptide vaccine. In silico analysis of Shp-1 showed the presence of B epitopes in the region 49-107. The polypeptide epitopic region Shp-149-107 was cloned and expressed in Escherichia coli. Antibody reactivity of the Shp-149-107 construct was evaluated in filarial endemic population by ELISA. Putatively immune endemic normals (EN) showed significantly high reactivity (P < 0.05) when compared to all the other categories. Antibody reactivity of Shp-1 repeat region was similar to that of whole protein proving that this region carries B epitopes responsible for its humoral response in humans. Thus this can be employed for inducing anti-microfilarial immunity in the infected population that may lead to reduction in transmission intensity and also it could be used along with other epitopes from different stages of the parasite in order to manage the disease effectively.
DOI: 10.2478/s11686-014-0270-y
PubMed: 25119360
Affiliations:
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Le document en format XML
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type="abstract" xml:lang="en">Transmission of lymphatic filariasis is mediated through microfilariae (L1 stage of the parasite) which is encased in an eggshell called sheath. The sheath protein Shp-1 stabilizes the structure due to the unique repeat region with Met-Pro-Pro-Gln-Gly sequences. Microfilarial proteins could be used as transmission blocking vaccines. Since the repeat region of Shp-1 was predicted to carry putative B epitopes, this region was used to analyze its reactivity with clinical samples towards construction of peptide vaccine. In silico analysis of Shp-1 showed the presence of B epitopes in the region 49-107. The polypeptide epitopic region Shp-149-107 was cloned and expressed in Escherichia coli. Antibody reactivity of the Shp-149-107 construct was evaluated in filarial endemic population by ELISA. Putatively immune endemic normals (EN) showed significantly high reactivity (P < 0.05) when compared to all the other categories. Antibody reactivity of Shp-1 repeat region was similar to that of whole protein proving that this region carries B epitopes responsible for its humoral response in humans. Thus this can be employed for inducing anti-microfilarial immunity in the infected population that may lead to reduction in transmission intensity and also it could be used along with other epitopes from different stages of the parasite in order to manage the disease effectively.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Madhumathi, Jayaprakasam" sort="Madhumathi, Jayaprakasam" uniqKey="Madhumathi J" first="Jayaprakasam" last="Madhumathi">Jayaprakasam Madhumathi</name><name sortKey="Prince, Rajaiah Prabhu" sort="Prince, Rajaiah Prabhu" uniqKey="Prince R" first="Rajaiah Prabhu" last="Prince">Rajaiah Prabhu Prince</name></noCountry><country name="Inde"><noRegion><name sortKey="Jawaharlal, Jeya Prita Parasurama" sort="Jawaharlal, Jeya Prita Parasurama" uniqKey="Jawaharlal J" first="Jeya Prita Parasurama" last="Jawaharlal">Jeya Prita Parasurama Jawaharlal</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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